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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Exp Eye Res. 2008 May 16;88(2):140–143. doi: 10.1016/j.exer.2008.05.005

Fig. 2.

Fig. 2

Selective activation of Src family members following ATP treatment. Rabbit lenses were incubated for 5 minutes in the presence or absence (control) of 100μM ATP then the epithelium was isolated from each lens and lysed. Yes, Src or Fyn protein was isolated from the lysate by immunoprecipitation, subjected to electrophoresis on an SDS-polyacrylamide gel, transferred to nitrocellulose and probed for active Src family kinases with an antibody against Src family kinases phosphorylated at the active loop tyrosine. Total Yes, Src, or Fyn protein was probed as loading control. Src and Fyn phosphorylation increased significantly, pointing to activation of these tyrosine kinases following ATP treatment of the lens. The data are the mean ± SE (vertical bar) of results from 12, 9 and 7 pools of lysates for Yes, Src and Fyn, respectively. * Indicates significant difference from control (p < 0.05).From (Tamiya et al., 2007); permission requested.