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Western blot analysis of equivalent amounts of total protein from transgenic (Tg) and non-transgenic (Ctl), enteric neuromuscular samples shows no differences in the amount of nNOS or ChAT at 4 weeks of age. In extracts from 15-week mice, the amount of nNOS in the Tg samples is dramatically reduced relative to Ctl samples. However, the equivalent amounts of ChAT are detected in extracts from each genotype.
