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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: J Immunol. 2006 Jul 15;177(2):925–933. doi: 10.4049/jimmunol.177.2.925

Figure 5.

Figure 5

The suboptimal ability to activate naïve T cells at seven days following L. major infection is not dependent upon immunosuppression. (A) BALB/c IL-10−/− recipient mice (Thy1.2+) were infected with L. major and CFSE-labeled ABLE transgenic T cells (Thy1.1+) were transferred at one or seven days following infection. As controls, experiments were also performed with wild type recipient mice as described in Fig. 1. Dot plots are gated on donor cells in the dLNs of recipient mice (Thy1.1+). The numbers in the corners of the dot plots represent the percentage of events in either of the CFSEdim quadrants, and the numbers in parentheses represent the percentage of cells that produce IFN-γ that are CFSEdim. (B) BALB/c recipient mice (Thy1.2) were depleted of Tregs by administration of 1mg of anti-CD25 (PC61) antibody intraperitoneally 7 days prior to infection, and control mice were treated with 1mg of rat IgG. Following infection, CFSE-labeled ABLE transgenic T cells (Thy1.1+) cells were transferred, harvested, and analyzed as described in Fig. 1. Dot plots were gated on donor cells in the dLNs of recipient mice (Thy1.1+).