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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: J Immunol. 2006 Jul 15;177(2):925–933. doi: 10.4049/jimmunol.177.2.925

Figure 8.

Figure 8

Naïve and antigen-experienced T cells compete for access to antigen presenting cells. Naïve BALB/c mice (Thy1.2+) were either infected with 5e6 stationary phase promastigotes or left uninfected. Naïve CFSE-labeled ABLE transgenic T cells (Thy1.1+) were transferred into naïve or d7 infected mice. One day after transfer LPS matured BMDDC pulsed with 5ug of LACK peptide or left unpulsed were adoptively transferred directly into the recipient via the footpad. Three days following transfer of BMDDC, cells were harvested and analyzed by flow cytometry. Histogram plots were gated on donor cells (Thy1.1+). The number in the corner represents the percentage of donor cells that were CFSEdim in the dLNs. Non-draining lymph nodes were also harvested and analyzed for proliferation (insets). The numbers in the corners of the dot plots indicated the percentage of donor cells that acquired the ability to produce IFN-gamma in the dLN of the recipient animals. The data are representative of two experiments.