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. 2009 Mar 25;29(12):3749–3759. doi: 10.1523/JNEUROSCI.5404-08.2009

Figure 1.

Figure 1.

A, B, Evoked EPSCs elicited from ciliary neurons have contributions from both α3-nAChRs and α7-nAChRs. C, D, α3-nAChR- and α7-nAChR-mediated currents show inward rectification. A1 and B1 show examples of EPSCs elicited by 0.03 Hz preganglionic stimulation before and after addition of either 300 nm α-CTx MII (A1), which blocks α3-nAChRs, or 50 nm MLA (B1), which blocks α7-nAChRs. A2 reproduces the EPSC from A1 in α-CTx MII (α7-nAChR current) plus a difference curve (before minus after, representing α3-nAChR current), whereas B2 reproduces the EPSC in B1 in MLA (α3-nAChR current) plus a difference curve (representing α7-nAChR current). α3-nAChR currents are slower than α7-nAChR currents (A2, B2). All records are the average of 3–5 traces. C shows an EPSC from one connection at holding potentials of −60 mV to +60 mV (15 mV increments), and D shows the current at the peak (dominated by α7-nAChRs) and 10 ms after the peak (dominated by α3-nAChRs) versus holding potential, uncorrected for a calculated −5 mV liquid junction potential. Both α7-nAChR- and α3-nAChR-mediated currents rectify to approximately the same degree. Each trace in C is the average of three traces collected at 0.03 Hz. Similar results were obtained in three other cells.