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. 2009 May 29;10:53. doi: 10.1186/1471-2199-10-53

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Copyright © 2009 Ernst and Noebels; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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tottering mutant mice display unaltered Cacna1g mRNA alternative splicing within the brain. Compared to wildtype littermates (A), tottering mutants (B) exhibit similar patterns of alternative splicing of Cacna1g transcripts assayed by RT-PCR from whole brain samples. Lanes within the agarose gel that are labelled (1–10) represent portions of the Cacna1g gene that undergo alternative splicing, while lanes without labels demonstrate regions of the gene not regulated by alternative splicing. Lane assignments: C, α-tubulin amplification loading control; 1, Δ5' E2; 2, +I6-7 & Δ5' E8; 3, Δ3' E8; 4, ΔE12; 5, ΔE14; 6, Δ3' E25; 7, ΔE26; 8, Δ5' E31; 9, Δ5' E34/ΔE34; 10, ΔE35. Alternative splice variants are denoted by the arrows.