Table 3.
Binding constants Ka (M−1), determined by fluorescence titration, and deduced free energy of association −ΔGO (kcal/mol, in parentheses) for IgGs specific for LPS of V. cholerae O1 serotype Ogawa, with synthetic methyl α-glycosides of fragments of Ogawa O-SP (8)
| IgG | Synthetic fragments of the Ogawa O-SP
|
|||||
|---|---|---|---|---|---|---|
| Monomer (1) | Dimer (2) | Trimer (3) | Tetramer (4) | Pentamer (5) | Hexamer (6) | |
| S-20-4 | 3.9 × 105 (31.9) | 1.2 × 106 (34.7) | 8.5 × 105 (33.8) | 1.6 × 106 (35.4) | 1.1 × 106 (34.5) | 1.3 × 106 (34.9) |
| A-20-6 | 1.9 × 105 (30.1) | 5.8 × 105 (32.9) | 7.7 × 105 (33.6) | 1.5 × 106 (35.2) | 9.7 × 105 (34.2) | 1.0 × 106 (34.2) |