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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Exp Cell Res. 2009 Mar 21;315(13):2231–2240. doi: 10.1016/j.yexcr.2009.03.008

Fig. 4. Minimal Sox9 binding site in the Bapx1 promoter in chondrocytes.

Fig. 4

a. Sequence of the Bapx1 promoter between −989 and −775 from the TSS. A potential Sox9 binding site is indicated in red. b. A luciferase reporter construct containing the Bapx1 minimum promoter is shown. Numbers indicate the position from the TSS. c. C3H10T1/2 cells were co-transfected with 200 ng of the indicated luciferase reporter plasmid and specified amounts of the plasmid-borne Sox9. Mean values (n= 3) of luciferase reporter activity ± standard deviation are shown. d. Chromatin-immunoprecipitated Sox9-binding non-enriched (rabbit IgG) DNA, enriched (Sox9) DNA, and 0.01% whole cell extract (WCE) DNA in chondrocytes was amplified by PCR using Sox9 site-specific primer sets for the Sox9 putative binding site, chondrocyte-specific target Col2a1, and Sertoli cell-specific target Amh.