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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Exp Cell Res. 2009 Apr 18;315(13):2207–2214. doi: 10.1016/j.yexcr.2009.04.008

Figure 4.

Figure 4

Fibronectin assembly is mediated by Integrin α5β1 in Null and Null + cells: A) Exogenous assembly assay; Cells were plated on 10ug/ml fibronectin or 5 ug/ml vitronectin in fibronectin free medium prior to the addition of biotinylated fibronectin. Cells were incubated overnight followed by collection of lysates and separation into DOC-soluble and insoluble fractions. Left panels: DOC-insoluble fractions. Biotinylated fibronectin is detected by blotting with IR Dye 680 Streptavidin. Vimentin serves as a loading control. Right Panels: DOC-soluble fraction: Actin serves as a loading control. B) Densitometric analysis of DOC insoluble fraction in panel A. Gray bars represent Null cells. White bars represent Null + cells. C) Hanging drop assay: Cells were treated as in figures 1 and 2 except for treatment with 100 μg/ml inhibitory antibodies against integrins β1 (HA 2/5), β3 (2C9.G2) or a combination of both.