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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Exp Cell Res. 2009 Apr 18;315(13):2207–2214. doi: 10.1016/j.yexcr.2009.04.008

Figure 5.

Figure 5

Comparison of fibronectin mRNA and protein levels: A) QRT-PCR of RNA extracted from Null and Null + cells. B) Null and Null + cells were plated and cultured overnight. Cell lystates were harvested in RIPA buffer and conditioned medium (CM) was collected. Equal amounts of protein were resolved by SDS-PAGE followed by detection with rabbit FN and mouse actin antibodies. C) Cells were pulsed for 10 minutes with 35S methionine followed by lysis and Immunoprecipitation for fibronectin. Abbreviations FN Fibronectin; TCL total cell lysate D) Null and Null + were pulsed for 35S methionine followed by a two hour chase. The levels of radioactivity were determined using scintillation counter and normalized to cell lysate protein levels.