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. 2009 Jun 24;4(6):e6027. doi: 10.1371/journal.pone.0006027

Figure 3. Satellite cell IL-6 protein expression following muscle lengthening contractions (MLC).

Figure 3

(3a) Il-6+ satellite cells expressed as a percentage of all Pax7+ satellite cells following MLC. (3b) Il-6+ satellite cells expressed as a percentage of total myonuclei following MLC. Values are reported as mean±S.E.M. Mean values represent the mean for all 8 subjects per time-point (8 samples per time-point, 40 samples total). *p<0.05 vs. 0 hr; †p<0.05 vs. 4 hr. (3c)IL-6/Pax7 co-localization following muscle lengthening contractions (MLC). Triple-immunofluorescent staining of 7 µm muscle cross-sections for satellite cells (red = Pax7+), IL-6 protein (green) and nuclei (DAPI = blue). The thin arrow denotes a Pax7+/IL-6+ cell and the thick arrows denote Pax7+ cells with no IL-6 staining (scale bar = 10 µm). (3d) Representative image of 72 hr (T72) where white arrows denotes a Pax7+ cells with no IL-6 staining (scale bar = 20 µm).