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CD26-binding protein p300 is M6P/IGFIIR. (A and B) CD26-binding protein p300 was immunoprecipitated from surface-biotinylated K562 cells as described in Fig. 1B. After SDS/PAGE under reducing conditions and transfer to a membrane, p300 was detected with streptavidin (A). After stripping, the membrane was probed with rabbit anti-M6P/IGFIIR polyclonal antibody (B).
