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. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: J Immunol. 2008 May 1;180(9):6346–6353. doi: 10.4049/jimmunol.180.9.6346

FIGURE 5.

FIGURE 5

Detection of human cytokines in cell supernatants. Cytokine production was detected in cell supernatants when immortalized Gal+/+ and Gal−/− PAECs were incubated with human whole blood (HWB) alone or with complement inhibitors C5aR antagonist (C5aRa, 14 µM) or compstatin (comp., 100 µM). A, Human cytokines (IL-6, IL-8, IFN-γ, and TNF-α) for which cross-reactivity to porcine cytokines was excluded in the human multiplex system (Table II). B, Human cytokines (MIP-1α, MIP-β, eotaxin, and IP-10) for which cross-reactivity with porcine cytokines could not be determined. Significant differences were found between human cytokines detected in the Gal+/+ compared with Gal−/− PAECs for all cytokines (*, p = 0.03). Data are presented as box plots showing median, quartiles, and minimum and maximum values from five experiments, each using a different donor.