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Affinity binding of Cramoll 1, 4 on FMZAG-12L. An amount of 1.5 mg of Cramoll 1, 4 was incubated with the magnetic particles and eluted with buffer and glucose afterwards (arrow). (a) Fractions (1 mL) were collected and their absorbance at 280 nm established (a). Each bar represents the mean ± SD of four experiments using the same FMZAG-12L particles. (b) Electrophoresis to native and basic protein (100 μg) from the 14th and 15th fractions, stained with Amido Black.
