Figure 4.

Affinity binding of F40–60 on FMZAG-12L. Aliquots of 18 mg (■), 10 mg (•) and 5 mg (∘) were incubated with the magnetic particles and eluted with buffer and glucose (arrow). (a) Fractions (1 mL) were collected and their absorbance at 280 nm measured (a). Each bar represents the mean ± SD of four experiments using the same FMZAG-12L particles when 10 mg of F40–60 were used. (b) Electrophoresis to native and basic protein (100 μg) from the 10 mg protein F40–60 profile of the 10th and 11th fractions was stained with Amido Black.