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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Inflamm Bowel Dis. 2009 May;15(5):697–706. doi: 10.1002/ibd.20827

FIGURE 5.

FIGURE 5

Detection of MAdCAM-1 and GlcNAc6ST transcripts by RT-PCR. Total RNA was prepared from FFPE tissue sections with UC in active (N = 5) and remission (N = 5) phases along with normal colonic mucosa (N = 5). MAdCAM-1 is constitutively expressed in UC, regardless of disease activity (4/5 samples in both active and remission phases) as well as in normal colonic mucosa (5/5 samples). GlcNAc6ST-1 is transcribed in all patient samples of active UC and normal colonic mucosa, but in three of five samples with UC in remission phase. GlcNAc6ST-2 transcripts are detected in only one of five patient samples from UC in active phase and two of five normal colonic mucosa samples. Cont., control amplification using distilled water (-) and plasmid harboring the target cDNA (+).