Skip to main content
. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Inflamm Bowel Dis. 2009 May;15(5):697–706. doi: 10.1002/ibd.20827

FIGURE 6.

FIGURE 6

Double immunofluorescence of Lec2 cells transiently transfected with either CD34, MAdCAM-1, or pcDNA1 (mock) vectors together with Core1-β3GlcNAcT cDNA and either GlcNAc6ST-1 (middle panels), GlcNAc6ST-2 (right panels), or pcDNA1 (mock) (left panels) vectors to detect core proteins (CD34 or MAdCAM-1) (red) and the MECA-79 epitope (green) simultaneously. GlcNAc6ST-2 (right panels) transfers sulfate to N-acetyllactosamine attached to extended core 1 O-glycans to form the MECA-79 epitope on all core proteins examined, including endogenous Lec2 cell membrane proteins, without apparent substrate preference for core protein. On the other hand, GlcNAc6ST-1 (middle panels) does not transfer sulfate to form the MECA-79 epitope on either CD34 or Lec2 membrane proteins, but does to MAdCAM-1. Bar = 50 μm.