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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Inflamm Bowel Dis. 2009 May;15(5):697–706. doi: 10.1002/ibd.20827

FIGURE 8.

FIGURE 8

Western blotting analysis of Protein A-Sepharose-purified glycoproteins CD34•IgG and MAdCAM-1•IgG obtained from the culture medium of Lec2 cells transiently transfected with either pcDNA1-CD34•IgG or pcDNA1-MAdCAM-1•IgG combined with pcDNA3-Core1-β3GlcNAcT and either pcDNA1-GlcNAc6ST-1, pcDNA1-GlcNAc6ST-2, or pcDNA1 (mock). GlcNAc6ST-2 robustly transfers sulfate to form the MECA-79 epitope on both scaffold proteins (CD34 and MAdCAM-1). On the other hand, GlcNAc6ST-1 very weakly but specifically forms MECA-79 epitope on MAdCAM-1 but not on CD34 (arrowhead).