Figure 2.1.

Degradation of E. coli 23S and16S rRNAsby RNase R from Mycoplasma genitalium (MgR). rRNAs isolated from ribosomes (see text) were incubated with buffer alone for 120 min, or with 0.5 μg of MgR in a 10-μl reaction for 30, 60, or 120 min under conditions described previously (Lalonde et al., 2007). The resulting products were separated in a 1.5% agarose gel, stained with SYBR Gold, and visualized under UV light. The starting 23S and 16S RNAs are labeled on the left. Prominent degradation intermediates are labeled with bars on the right.