Table 1.
Chemosensitivity testing, constitutive protein, and mRNA expression of BCRP, LRP, MDR1, and MRP1 in 26 xenografts and the positive controls. Response: − negative: 100–50% T/C, + 35–50% T/C, ++ 21–35% T/C, +++ 6–20%T/C, ++++ 0–5% T/C, tox-toxic, n.t.—not tested; protein expression: − not detected, + weak, ++ medium, +++ strong expression; % of positive cells; mRNA expression: normalised ΔCT values; etp—etoposide, carpl—carboplatin, gem—gemcitabine, paltx—paclitaxel, erlo—erlotinib.
| Before treatment | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chemosensitivity | Protein expression | mRNA expression | |||||||||||
| LuCa | etp | Carpl | gem | paltx | erlo | BCRP | LRP | MDR1 [%] | MRP1 [%] | bcrp | lrp | mdr1 | mrp1 |
| 7064 | ++ | − | − | ++ | ++ | − | + | 15.4 | 13.8 | 12.19 | 8.01 | 17.54 | 7.10 |
| 7126 | − | − | +++ | − | ++ | +++ | + | 15.3 | 18.2 | 4.17 | 5.04 | 18.18 | 3.45 |
| 7166 | ++ | ++ | + | − | − | − | +++ | 13.7 | 13.8 | 15.46 | 6.45 | 0 | 7.16 |
| 7177 | − | ++ | +++ | − | ++ | − | ++ | 14.5 | 29.5 | 10.42 | 5.33 | 16.97 | 2.02 |
| 7187 | − | ++ | +++ | − | − | − | ++ | 15.4 | 18.3 | 8.13 | 6.90 | 0 | 7.55 |
| 7198 | − | + | + | + | − | +++ | ++ | 27.2 | 20.9 | 10.13 | 6.18 | 21.24 | 6.25 |
| 7298 | + | + | ++ | ++ | − | − | ++ | 14.4 | 13.1 | 15.03 | 5.79 | 17.19 | 6.33 |
| 7336 | − | (+) | ++ | +++ | − | +++ | + | 13.3 | 13.6 | 8.08 | 6.68 | 0 | 7.02 |
| 7343 | − | +++ | +++ | ++ | − | + | + | 20.8 | 24.1 | 8.34 | 5.85 | 0 | 5.23 |
| 7387 | − | − | +++ | ++++ | − | ++ | ++ | 15.4 | 29.9 | 6.15 | 7.64 | 10.90 | 7.57 |
| 7406 | + | + | +++ | +++ | − | +++ | + | 29.4 | 22.4 | 14.87 | 7.61 | 14.97 | 8.10 |
| 7414 | − | ++ | +++ | +++ | + | − | ++ | 17.9 | 15.6 | 7.13 | 5.69 | 0 | 6.21 |
| 7433 | − | +++ | − | ++++ | − | − | +++ | 18.8 | 22.1 | 6.99 | 5.68 | 17.20 | 3.73 |
| 7462 | − | + | ++++ | +++ | ++ | + | − | 39.8 | 31.3 | 7.65 | 5.24 | 9.36 | 7.78 |
| 7466 | − | − | ++++ | ++++ | ++ | + | ++ | 15.5 | 18.6 | 8.62 | 7.56 | 22.43 | 8.65 |
| 7506 | − | ++++ | (+) | +++ | − | + | + | 65.8 | 19.0 | 10.96 | 7.07 | 15.94 | 6.98 |
| 7530 | ++++ | − | tox | +++ | − | +++ | + | 20.1 | 16.1 | 14.27 | 8.24 | 14.35 | 8.01 |
| 7558 | − | ++++ | +++ | − | − | +++ | ++ | 24.0 | 25.3 | 15.90 | 6.26 | 18.64 | 6.38 |
| 7612 | − | ++++ | − | +++ | − | − | + | 17.7 | 15.5 | 11.09 | 5.89 | 16.98 | 6.04 |
| 7668 | − | +++ | tox | ++++ | − | +++ | − | 17.2 | 24.8 | 7.55 | 6.12 | 13.42 | 6.17 |
| 7700 | − | − | ++++ | ++ | − | ++ | +++ | 18.1 | 13.9 | 15.81 | 6.01 | 17.32 | 7.96 |
| 7747 | − | ++ | − | ++ | − | + | ++ | 62.0 | 15.0 | 15.19 | 4.82 | 18.29 | 6.26 |
| 7766 | − | +++ | + | ++ | − | + | + | 33.1 | 18.0 | 6.72 | 5.18 | 17.88 | 4.14 |
| 7860 | + | − | ++ | +++ | − | + | ++ | 20.6 | 22.4 | 11.16 | 4.96 | 16.41 | 5.47 |
| 7913 | − | + | (+) | +++ | − | + | ++ | 14.0 | n.t. | 13.50 | 5.64 | 18.47 | 6.17 |
| 7915 | n.t | n.t | n.t | n.t | − | + | ++ | 16.0 | 13.8 | 14.27 | 8.49 | 16.21 | 6.87 |
|
| |||||||||||||
| MDA-MB-231/BCRP | 2.51 | ||||||||||||
| A549 | 9.97 | 5.54 | |||||||||||
| MT3/ADR | 3.49 | ||||||||||||