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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Bioorg Med Chem. 2009 May 3;17(11):3770–3774. doi: 10.1016/j.bmc.2009.04.057

Figure 4.

Figure 4

Photoaffinity labeling of DAT with [125I]-6. Cells expressing rDAT or 6X-his- hDAT were photoaffinity-labeled with [125I]-6 in the absence or presence of 100 μM (−)-cocaine. Cells were solubilized and DATs were immunoprecipitated with DAT antibody 16 (rDAT) or anti-his antibody (hDAT) followed by SDS-PAGE and autoradiography.