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. 2000 Jul 11;97(15):8445–8450. doi: 10.1073/pnas.150236797

Figure 1.

Figure 1

Presentation of lipid antigen to the CD1c-restricted CD8–1/J.RT3 cells. (A) Untransfected JY cells and JY cells stably transfected with either CD1a (JY/CD1a), CD1b (JY/CD1b), or CD1c (JY/CD1c) were incubated overnight either with or without 1.56 μg/ml of chloroform/methanol extract of M.tb H37Ra sonicate, fixed with 0.08% glutaraldehyde, and used as antigen-presenting cells. The J.RT3 transfectants expressing the TCR α and β chains derived from CD8–1 (CD8–1/J.RT3) were cultured with these fixed antigen-presenting cells for 24 h in the presence of phorbol 12-myristate 13-acetate (10 ng/ml), and IL-2 production was measured, using the HT-2 indicator cells. (B) Monocyte-derived dendritic cells were incubated for 4 h with the chloroform/methanol extract of H37Ra sonicate (5 μg/ml) either before or after fixation with 0.04% glutaraldehyde. The cells were then washed and incubated with CD8–1/J.RT3, and IL-2 release into the media was measured.