Figure 3.

Colocalization of CD1a, -b, and -c with LAMP-1. Monocyte-derived dendritic cells were fixed and permeablized after a cytospin procedure. The cells were then double-labeled with mouse mAbs to CD1a (A), CD1b (D), or CD1c (G) (detected with Texas Red-conjugated donkey anti-mouse IgG) and a rabbit antiserum against human LAMP-1 (B, E, and H) (detected with FITC-conjugated donkey anti-rabbit IgG) and analyzed by confocal microscopy. The corresponding red and green fluorescent confocal images then were superimposed to detect any cellular compartments expressing both CD1 and LAMP-1 (C, F, and I, yellow vesicles). (Scale bars = 5 μm.)