Table 2. Effects of CPT-11 treatment and Cipro on phenotypic distribution of immune cells in MLN and spleen.
MLN
|
Spleen
|
|||||
---|---|---|---|---|---|---|
Antibody | Healthy controls n=8 | CPT-11 alone n=9 | CPT-11+Cipro n=6 | Healthy controls n=8 | CPT-11 alone n=9 | CPT-11 + Cipro n=6 |
B cell+(OX12) (% of total cells) | 13.0±0.6a | 19.8±1.8b | 27.3±1.9c | 29.1±0.4a | 36.4±1.8b | 36.9±1.1b |
% OX12+CD45RA− | 1.5±0.3a | 5.3±0.6b | 5.0±1.7b | 7.5±0.8a | 28.7±3.0b | 8.3±0.9a |
% OX12+CD45RA+ | 98.5±0.3a | 94.7±0.6b | 95.0±1.7b | 92.5±0.8a | 71.3±3.0b | 91.7±0.9a |
% OX12+CD80+ | 0.7±0.1a | 1.5±0.5a,b | 2.6±1.1b | 2.8±0.4 | 3.9±0.7 | 2.5±0.3 |
CD3+(% of total cells) | 70.8±1.2a | 66.4±1.4b | 57.5±1.2c | 55.4±0.6a | 46.4±2.1b | 49.6±0.8b |
CD3+CD8+(% of total cells) | 22.2±0.8a | 23.0±0.6a | 17.9±0.5b | 31.9±0.9a | 21.0±0.9b | 23.9±1.1b |
% CD8+CD71+ | 9.2±1.2a | 10.5±1.0a | 19.3±2.6b | 25.6±0.8a | 23.9±1.2a | 28.8±1.3b |
% CD8+CD28+ | 4.0±0.6a | 50.8±4.5b | 75.2±2.9c | 10.0±0.5a | 52.3±6.3b | 44.5±9.3b |
% CD8+CD25+ | 9.3±0.3a | 9.4±1.0a | 40.2±9.1b | 8.6±0.8a | 16.4±3.7b | 14.0±1.2b |
% CD8+CD62 L+ | 38.0±2.0a | 42.8±3.1a | 30.1±2.4b | 33.8±0.9a | 34.7±3.1a | 15.7±0.6b |
% CD8+CD45RA− | 62.4±1.7a | 76.7±6.5b | 38.4±2.5c | 31.8±1.2a | 45.9±4.6b | 28.1±1.5a |
% CD8+CD45RA+ | 37.6±1.7a | 23.3±6.5b | 61.6±2.5c | 68.2±1.2a | 54.1±4.6b | 71.9±1.5a |
CD3+CD4+(% of total cells) | 48.7±0.7a | 43.3±1.4b | 40.7±1.2b | 32.4±0.5a | 22.2±1.0b | 20.4±1.7b |
% CD4+CD71+ | 6.5±0.7a | 9.2±1.2b | 4.4±0.9a | 22.1±1.1a | 39.9±3.8b | 26.7±2.1c |
% CD4+CD28+ | 5.5±0.3a | 73.9±5.1b | 85.2±2.2b | 15.2±0.9a | 60.7±10.4b | 77.6±2.5b |
% CD4+CD25+ | 7.5±0.4a | 9.8±0.7b | 37.3±11.9c | 9.4±0.4a | 15.4±2.2b | 10.8±0.9a,b |
% CD4+CD62L+ | 74.7±4.2a | 53.3±6.5b | 59.4±4.4b | 67.8±2.1a | 36.1±4.7b | 28.4±1.0b |
% CD4+CD45RA− | 93.8±0.7a | 91.9±3.4a | 90.6±0.5b | 80.2±0.5a | 79.9±2.0a,b | 83.4±1.3b |
% CD4+CD45RA+ | 6.2±0.7a | 8.1±3.4a,b | 9.4±0.5b | 19.8±0.5a | 20.1±2.0a,b | 16.6±1.3b |
CD3+CD4+/CD3+CD8+ | 2.2±0.1a | 1.9±0.0b | 2.3±0.1a | 1.0±0.0a | 1.1±0.0a | 0.9±0.1b |
CD71 (% of total cells) | 15.8±0.7a | 12.8±0.9b | 14.6±0.6a,b | 23.5±0.8a | 33.5±2.6b | 22.6±2.1a |
CD25 (% of total cells) | 4.3±0.2a | 5.7±0.4b | 27.1±7.5c | 5.5±0.5a | 10.2±1.9b | 6.5±0.6a,b |
CD28 (% of total cells) | 3.4±0.2a | 44.8±3.2b | 55.4±1.9c | 7.4±0.5a | 29.3±2.9b | 38.5±3.5b |
CD62 L (% of total cells) | 48.2±2.8a | 46.7±2.5a | 35.3±1.1b | 39.3±1.2a | 31.4±1.4b | 15.3±0.5c |
CD45RA+(% of total cells) | 36.3±1.3a | 24.8±1.7b | 35.0±1.6a | 56.3±0.5a | 36.4±3.2b | 52.0±1.1c |
Data presented as cell population percentage mean±s.e.m.; means within a row for a given cell type (splenocytes, mesenteric lymph node (MLN) cells) that do not share a common letter are significantly different (P<0.05). a,b,cMeans within a column that do not share a common letter are significantly different.