Table 3. Effects of CPT-11 treatment and Cipro on in vitro proliferation in response to Con A by immune cells in MLN and spleen.
|
MLN
|
Spleen
|
||||||
|---|---|---|---|---|---|---|---|
| Mitogen | Unit | Healthy controls | CPT-11 alone | CPT-11+Cipro | Healthy controls | CPT-11 alone | CPT-11+Cipro |
| 24 hours | |||||||
| None | DPM | 668±51 (8)a | 1359±143 (9)b | 482±65 (8)c | 2954±75 (8)a | 11204±1046 (9)b | 3498±348 (8)a |
| 48 hours after mitogen stimulation | |||||||
| None | DPM | 317±53 (8)a | 1023±128 (5)b | 251±57 (7)a | 5001±588 (8)a | 5603±840 (9)a | 3789±778 (8)a |
| CON A | SI | 35±10 (8)a | 104±16 (5)b | 29±11 (7)a | 8.70±1.50 (8)a | 0.10±0.02 (9)b | 1.05±0.33 (8)c |
SI=stimulation index.
a
Data are presented as mean±s.e.m. (number of rats), means within a row for a given cell type (splenocytes, mesenteric lymph node (MLN) cells) that do not share a common letter are significantly different (P<0.05).
b
Simulation index. a,b,cMeans within a column that do not share a common letter are significantly different.