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. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: Eur J Immunol. 2009 Apr;39(4):1167–1175. doi: 10.1002/eji.200839188

Figure 3.

Figure 3

Binding of LAIR-1 and Lilrb4 to collagen or collagen related peptide. (A) Soluble monomeric mLAIR-1/rCD4d3+4 (43 μM) was applied to collagen (2000 RU), collagen related peptide trimer, (GPO)10 (2000 RU), control peptide trimer (GPP)10 (2000 RU) and negative control streptavidin (1000 RU) coated flow cells. (B, C) Binding curves for experiments as in (A) but varying the concentration of purified mLAIR-1/rCD4d3+4. Kd of 10 and 18 μM were calculated by nonlinear curve fitting of the Langmuir isotherm (line) to the binding data of monomeric mLAIR-1/rCD4d3+4 to (GPP)10 and collagen with the values for the negative control (GPP)10 subtracted. (D) and (E) Comparable experiment to (B) and (C) showing hLAIR-1/rCD4d3+4 and mLilrb4/rCD4d3+4 bound to collagen with Kd = 26 and 39 μM respectively.