Skip to main content
. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: Eur J Immunol. 2009 Apr;39(4):1167–1175. doi: 10.1002/eji.200839188

Figure 5.

Figure 5

hLAIR-1 gives comparable bead binding to mLilrb4 as that between mCD200 and mCD200RLa. mLilrb4/rCD4d3+4, mCD200RLa/rCD4d3+4, hLAIR1/rCD4d3+4 and rCD4d3+4 were immobilized on four flow cells and then three conjugated sets of beads were injected sequentially. (A) hLAIR-1 beads gave strong binding to mLilrb4 but not mCD200RLa or control rCD4d3+4 with traces on hLAIR-1. (B) mCD200RLa beads gave strong binding to its ligand CD200 but not the other proteins. (C) mLilrb4 beads gave minimal binding to mCD200 but very weak binding to the other proteins including the control protein. Inset table: the amount of multivalent beads coated with hLair1 and mLilrb4 that bound to surfaces with immobilized hLair1, mLair1, hLilrb4 and mLilrb4 from another experiment are listed.