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Time course analysis of pervanadate-induced tyrosine phosphorylation of PTK substrate proteins in scFv transfectants of J558L expressing scFv molecules with the wild-type Ig-α tail (lanes 1, 4, 7, 10, 13), the A1,2V-mutated Ig-α tail (lanes 2, 5, 8, 11, 14), or the tail truncation (lanes 3, 6, 9, 12, 15). Cells were stimulated for the indicated times with 8 μM pervanadate. The proteins of total cellular lysates were size-separated by SDS/10% PAGE and analyzed by 4G10 immunoblotting.
