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. 2000 Jul 18;97(15):8451–8454. doi: 10.1073/pnas.97.15.8451

Figure 4.

Figure 4

Influence on the A1,2T mutation of the Ig-α tail sequence on the phosphorylation of the two ITAM tyrosines. Western blot analysis of PTK substrate phosphorylation in J558L/Ig-α transfectants expressing either wild-type (wt) Ig-α (lanes 1 and 2) or mutations of Ig-α (lanes 3–16). The cells either were left unstimulated or were stimulated for 3 min with 20 μM pervanadate. The proteins of total cellular lysates were size separated by SDS/10% PAGE and analyzed by 4G10 immunoblotting.