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TCR point mutations that exhibited no measurable binding to pepMHC. The mutations produced during development of the ZAFFI filter are shown in italics.
| CDRα1 | CDRα3 | CDRβ1 | CDRβ3 |
|---|---|---|---|
| Q30M | T93Q | E30A | R95F |
| Q30H | T93I | E30F | L98D |
| Q30L | T98E | E30Q | L98F |
| Q30F | D99N | E30W | L98I |
| Q30W | S100M | E30Y | L98M |
| Q30Y | S100I | A99D | |
| S100L | A99Y | ||
| S100F | G100I | ||
| G102S | G101M | ||
| G102D | G101V |
