Table 4.

ZAFFI Scores, Filter Scores, and measured binding for all measured TCR point mutants.

Chain	Mutant	ZAFFI Score	ZAFFI Filter	Pass1	ΔΔG2
α	Q30W	−3.00	0.46	N	NB
α	Q30F	−2.90	0.63	N	NB
α	Q30Y	−2.37	0.66	N	NB
α	G28M	−1.88	0.03	Y	−0.51
α	Q30M	−1.82	0.63	N	NB
α	Q30H	−1.59	0.47	N	NB
α	Q30L	−1.50	0.63	N	NB
α	S100L	−1.49	0.08	N	NB
α	R27F	−1.28	0.15	N	−0.22
α	S100I	−1.07	0.08	N	NB
α	D26W	−1.05	0.03	Y	−1.08
β	G101A	−1.00	0.03	Y	−1.09
α	G28T	−0.97	0.01	Y	−0.76
α	S51M	−0.90	0.04	Y	−0.37
α	G28I	−0.89	0.03	Y	−0.27
α	G100I	−0.89	0.00	Y	NB
α	K68H	−0.83	0.93	N	1.09
β	A99M	−0.72	0.00	Y	−0.31
α	D26M	−0.49	0.03	Y	0.19
α	G28A	−0.46	0.03	Y	0.58
α	R95F	−0.46	0.33	N	NB
α	S100T	−0.46	0.01	Y	−0.49
α	G28L	−0.43	0.03	Y	−0.46
α	Q30E	−0.42	0.50	N	0.43
β	E30Y	−0.33	0.25	N	NB
β	E30F	−0.33	0.25	N	NB
β	E30W	−0.31	0.26	N	NB
β	G100S	−0.25	−0.02	Y	−0.32
α	S100A	−0.19	0.07	N	−0.01
β	E30M	−0.18	0.25	N	NB
α	T93I	−0.17	0.04	Y	NB
β	A99K	−0.14	0.05	N	0.1
α	S100M	−0.14	0.10	N	NB
α	S100F	−0.13	0.08	N	NB
α	G102D	−0.12	−0.11	Y	NB
α	D26V	−0.11	0.04	Y	1.45
β	A99D	−0.10	−0.01	Y	NB
α	G102S	−0.03	−0.61	Y	NB
β	R102Q	−0.03	0.14	N	0.45
α	T98E	−0.01	0.62	N	NB
α	S29A	0.00	0.02	Y	0.33
β	E30Q	0.01	0.21	N	NB
α	Q30N	0.10	−0.32	Y	0.73
β	E30A	0.10	0.25	N	NB
β	L98F	0.10	0.00	Y	NB
α	T93Q	0.22	−1.49	Y	NB
α	S100Y	0.26	0.22	N	2.29
β	L98I	0.49	0.00	Y	NB
β	G101V	0.51	0.03	Y	NB
α	G28V	0.52	0.03	Y	0.56
α	S100Q	0.52	0.10	N	NB
α	I54R	0.54	−0.74	Y	1.28
α	D99N	0.58	0.26	N	NB
α	S100N	0.58	0.11	N	1.92
β	G101M	0.61	0.02	Y	NB
β	L98M	0.96	0.01	Y	NB
α	G28R	1.65	−0.51	Y	2.43
β	A99Y	1.92	−0.01	Y	NB
β	L98D	2.48	0.09	N	NB

¹Whether the mutant passed the ZAFFI filter, defined by having a filter score less than 0.05.

²Measured binding energy change (kcal/mol). NB = no binding detected or unmeasurable binding.