. Author manuscript; available in PMC: 2010 May 30.

Published in final edited form as: J Neurosci Methods. 2009 Mar 5;180(1):116–125. doi: 10.1016/j.jneumeth.2009.02.014

Table 1.

Media components for serum media, Neurobasal media, 4% PFA, and 2% PFA/2.5 % glutaraldehyde. DMEM (Dulbecco’s Modified Eagle Medium), FBS (fetal bovine serum), PFA (paraformaldehyde), PB (phosphate buffer).

Serum Media	Neurobasal Media	4% PFA (pH = 7.0; store at 4°C)	2% PFA/2.5% Glutaraldehyde (pH = 7.4; store at 4°C)

DMEM (Gibco)–88%	Neurobasal media (Gibco)	PFA (Sigma) – 0.04 g/ml	PFA - 0.02 mg/ml
FBS (Gibco) –10%		Monobasic sodium phosphate (Sigma) – 2.64 mg/ml	Glutaraldehyde (EMS) (0.4M) – 10%
L-glutamine (Gibco) (0.2M) –1%		Dibasic sodium phosphate (Sigma) – 0.02 g/ml	Monobasic PB (0.2M) (0.28g/ml) – 10%
Antibiotic-antimycotic (Gibco) – 1%			Dibasic PB (0.2M) (0.28 g/ml) – 40%