Table 2.
Common primary antibodies used to study different human brain cell populations. A, Cell type and protein markers. Listed are the protein markers characteristic of different cell types. B, Catalogue numbers and dilutions of commonly used primary antibodies to identify these protein markers. C, Secondary antibodies to identify primary antibodies.
| A. | |
|---|---|
| CELL TYPE | PROTEIN MARKERS |
| Type B (Astrocyte) | GFAP, vimentin, nestin, S-100β |
| Type C (Transient Amplifiying Cell) | Nestin, Dlx2 |
| Type A (Migrating Neuroblast) | PSA-NCAM, Type III β-tubulin (Tuj1) |
| Ependymal cell | S-100β |
| Astrocytic lineage | GFAP |
| Neuronal lineage | NeuN, Map2, Tuj1 |
| Oligodendrocytic lineage | Olig-2, O4, S-100β |
| B. | ||||
|---|---|---|---|---|
| TARGET MOLECULE | MANUFACTURER | CATALOGUE NO. | HOST | DILUTION |
| GFAP | Chemicon | MAB3402 | Mouse | 1:500 |
| Dako | Z0334 | Rabbit | 1.500 | |
| Vimentin | Chemicon | MAB3400 | Mouse | 1:1000 |
| Nestin | Chemicon | MAB5326 | Mouse | 1:250 |
| S-100β | Sigma | HPA015768 | Rabbit | 1:500 |
| Dlx-2 | Chemicon | AB5726 | Rabbit | 1:500 |
| Neu-N | Chemicon | MAB377 | Mouse | 1:500 |
| Map-2 | Chemicon | AB5622 | Rabbit | 1:1000 |
| Tuj-1 | Chemicon | MAB1637 | Mouse | 1:250 |
| Olig-2 | Chemicon | AB9610 | Rabbit | 1:500 |
| O4 | Chemicon | MAB345 | Mouse | 1:500 |
| C. | ||||
|---|---|---|---|---|
| Target | Dye | Manufacturer | Catalogue No. | Dilution |
| Mouse | Alexa 488 | Molecular probes | A11001 | 1:500 |
| Mouse | Alexa 594 | Molecular probes | A11005 | 1:500 |
| Rabbit | Alexa 488 | Molecular probes | A11008 | 1:500 |
| Rabbit | Alexa 594 | Molecular probes | A11012 | 1:500 |