Skip to main content
. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: Mol Carcinog. 2009 Apr;48(4):369–378. doi: 10.1002/mc.20509

Figure 1. Validation of the RNA interference-based knockdown cellular models (A–C).

Figure 1

We generated U2OS clones expressing shRNA control sequences (shC1 and shC2) and targeting BACH1 (BRIP1) (shB1 and shB2), POL I (shι1 and shι2) or POL H (shη1 and shη2). Extracts from these cells were analyzed by immunoblotting with the indicated antibodies and Ku70 as a loading control (D) U2OS cells were transfected with control siRNA (Luciferase) or two independent Pol κ siRNAs (siκ1 and siκ2). The levels of Pol κ was analyzed by Western blotting 48h after transfection and normalized against actin.