Skip to main content
. 2000 Jul 11;97(15):8455–8460. doi: 10.1073/pnas.150240097

Figure 2.

Figure 2

Inhibition of T cell hybridoma recognition by γHV-68. (A) MEF-1 cells transduced either with pMSCV-ovalbumin-GFP retrovirus (MEF-OVA) or with pMSCV-GFP alone (MEF) were infected or not with γHV-68 (10 pfu per cell) and exposed or not to 1 μM SIINFEKL peptide as indicated. Six hours later, the MEF-1 cells were washed and mixed with B3Z hybridoma cells that recognize the SIINFEKL epitope of ovalbumin. β-Galactosidase production by B3Z in response to SIINFEKL presentation was then assayed with o-nitrophenyl galactopyranoside. (B) MEF-1 cells transduced with pMSCV-M-TSINFVKI-ASNENMETM-SIINFEKL-GFP polytope retrovirus (MEF-P) or with pMSCV-GFP alone (MEF) were infected or not with γHV-68 (10 pfu per cell). Six hours later, the MEF and MEF-P cells were washed three times and mixed in a 1:1 ratio as indicated before overnight culture with B3Z cells and assay for β-galactosidase production.