Figure 6.

Down-regulation of surface MHC class I expression by γHV-68 K3 and its KSHV homologs. The γHV-68 K3, the KSHV K3, and the KSHV K5 ORFs were cloned into pMSCV-GFP and expressed by retroviral transduction, as indicated in human 293 cells stably transfected with murine H-2K^b and murine L929 cells stably transfected with human HLA-A2. GFP⁺ transduced cells were then mixed with GFP⁻ untransduced cells to compare MHC class I expression, in each case using a phycoerythrin-conjugated goat anti-mouse IgG secondary antibody.