Skip to main content
. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: Science. 2007 Nov 2;318(5851):772–777. doi: 10.1126/science.1147710

Fig. 4.

Fig. 4

Delivery of nAG protein to regenerating newt limbs. (A) RFP expression at the end of the limb stump at day 10 pa, following electroporation at day 5 pa. (B) Expression of nAG in cells of the limb after electroporation of nAG plasmid at day 7 pa. The section was stained with antibodies to nAG (green). (C) Section of a nAG positive gland in the WE after electroporation of nAG plasmid into a denervated limb blastema at day 5 pa, and analysis at day 17 pa. (D) Experimental design for assaying activity of nAG on the denervated blastema. Newts were denervated and amputated, prior to electroporation on the denervated side with either vector or nAG plasmid DNA. (E) Representative animals at day 40 pa from the two groups of an experiment outlined in (D). The yellow star indicates the position of the initial denervation. Scale bars, B and C 250μm.