Figure 1.
Summary of LvDsh constructs used to identify regions required for vegetal, cortical localization (VCL). VCL scoring for each construct is shown. “+” indicates that VCL was evident in most embryos oriented favorably; “+/−” indicates that some, but not all, favorably oriented embryos exhibited VCL. For the “+/−” scored embryos, the crescent of GFP tagged protein in the vegetal, cortical region was generally less pronounced than that observed following injection of mRNA encoding wild-type LvDsh.GFP. “−” indicates that none of the embryos showed VCL. “NS” indicates that the embryos were not scored due to low levels of GFP fluorescence.