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. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: Dev Biol. 2007 Mar 6;306(1):50–65. doi: 10.1016/j.ydbio.2007.02.041

Figure 10.

Figure 10

The dominant negative construct, DIX, and its mutants are expressed. mRNA encoding DIX or one of its mutants tagged with a Myc epitope was injected at a concentration of 8 mg/ml. Forty late gastrula stage embryos from each injection were harvested, lysed in Laemmli sample buffer, separated by SDS-PAGE, and transferred to nitrocellulose. All proteins were detecting using a primary antibody against the Myc epitope tag. Forty, gastrula stage, uninjected embryos are shown as a control. Arrows indicate the doublet bands of LvDsh.DIX and LvDsh.DIX.K47A. Molecular weight standards are shown.