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. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: Dev Biol. 2007 Mar 6;306(1):50–65. doi: 10.1016/j.ydbio.2007.02.041

Figure 11.

Figure 11

LvDsh.DIX interacts via residues K57 and E58 with the DIX domain of full length LvDsh. LvDsh.GFP constructs were injected alone (A, D, and F) or co-injected with either nuclear-targeted LvDsh.DIX (B, E, and G) or nuclear-targeted mutant (K57A/E58A) LvDsh.DIX (C). Confocal images of representative embryos were taken at the blastula stage. Arrows indicate nuclei. When injected alone, wild-type LvDsh.GFP shows diffuse cytoplasmic staining with no nuclear accumulation (A). Co-injection with nuclear-targeted LvDsh.DIX results in a marked decline in cytoplasmic staining and a corresponding increase in nuclear LvDsh.GFP (B). The previously observed nuclear accumulation of LvDsh.ΔC.GFP is replicated when injected alone (D). Co-injection of LvDsh.ΔC.GFP with nuclear-targeted LvDsh.DIX greatly enhances the nuclear accumulation of the protein (E). The interaction between DIX and LvDsh is mediated by the DIX domain of the full-length protein, because LvDsh lacking the DIX domain (LvDsh.ΔDIX.GFP) shows minimal nuclear fluorescence in the absence (F) or presence (G) of nuclear-targeted LvDsh.DIX. Conserved, lipid-associating residues K57 and E58 within the DIX domain are required for its interaction with full length LvDsh.GFP (C).