ClustalW alignment of the DIX domain of Dishevelled (Dsh) from L. variegatus (Weitzel et al., 2004), X. laevis (Sokol et al., 1995), D. melanogaster (Klingensmith et al., 1994), M. musculus (Sussman et al., 1994), and H. sapiens (Pizzuti et al., 1996a). Defined motifs for lipid interaction and actin binding are boxed. An asterisk indicates the specific residues within each motif targeted for mutation.