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. Author manuscript; available in PMC: 2009 Jun 16.
Published in final edited form as: Dev Biol. 2007 Mar 6;306(1):50–65. doi: 10.1016/j.ydbio.2007.02.041

Figure 9.

Figure 9

The dominant negative phenotype produced by the DIX domain of LvDsh is not caused by the specific localization of LvDsh.DIX or inhibition of the nuclear accumulation of LvDsh. (A) Summary of constructs used to target LvDsh.DIX to the nucleus. (B) Brightfield image of a representative embryo overexpressing a nuclear-targeted form of LvDsh.DIX. Nuclearizing LvDsh.DIX has no effect on its ability to generate a dominant negative phenotype. (C) Confocal image of GFP fluorescence confirms the presence of the protein within the nuclei of cells of a blastula stage embryo. (D) Confocal image of GFP fluorescence confirms the presence of LvDsh.ΔC within the nuclei of cells of a blastula stage embryo. This embryo, co-injected with LvDsh.DIX, develops a typical dominant negative phenotype.