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. 2009 May 20;29(20):6526–6534. doi: 10.1523/JNEUROSCI.1429-09.2009

Figure 2.

Figure 2.

cytNmnat1 is present in axons. DRG neurons were infected with lentivirus expressing 6xHis epitope-tagged Nmnat1 or cytNmnat1. The infected neurons were stained with anti-6xHis antibody. Nmnat1 was predominantly expressed in the nucleus and cytNmnat1 is expressed diffusely in the cytosol (top row). cytNmnat1 was detected in axons at high levels, whereas no Nmnat1 was detected within the axon (middle row). Phase-contrast pictures corresponding to the axonal area stained with anti-6xHis antibody are shown (bottom row). Scale bar, 25 μm.