Figure 1.

Abelson lines are efficiently infected by retroviruses. (A) Schematic diagram of retroviral vectors used in this study. The MSCV LTR drives the expression of the enhanced Gfp downstream of an IRES in the control vector, MiGfp. The cre recombinase is cloned upstream of the IRES GFP cassette and also is driven by the MSCV LTR, MCiGfp. MCS, multiple cloning site. (B) Fluorescence-activated cell sorting analysis measuring Gfp expression of the Abelson line Dnmt^cond1 infected with either MiGfp or MCiGfp at 2 days postinfection gated on live cells by scatter properties. (C) Measurement of the percentage of live Gfp⁺ cells as a function of time postinfection either infected with MiGfp or MCiGfp as labeled. Each line represents an independent experiment.