Figure 2.

Abelson lines infected with MCiGfp efficiently excise the Dnmt1 locus. (A) Schematic diagram of the genomic Dnmt1 locus. The genotype of the Abelson lines are 2lox/N before cre-mediated excision. WT, wild type. (B) Southern blot analysis of the Dnmt1 locus from purified populations of infected Dnmt^cond Abelson lines infected with either MiGfp or MCiGfp and sorted for Gfp⁺ viable cells (G⁺ or CiG⁺, respectively). The DNA was digested with SpeI and probed with a fragment from the Dnmt1 locus. The bands are labeled on the right.