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Targeted expression of cameleon indicators in NE/ER and nucleoplasm. (A) NG108–15 cells and (B) Jurkat cells were transfected with ER- or Nu-cameleon. ER-cameleon-expressing cells were immunostained with antinuclear pore protein (NPP) Ab and second antibody with Texas red, showing expression of cameleon protein in the NE and throughout the ER. Nu-cameleon-expressing cells were fixed and stained with nuclear-specific dye Hoechst 33342, showing restricted expression in the nucleoplasm.
