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. 2009 Jun 12;4(6):e5868. doi: 10.1371/journal.pone.0005868

Figure 4. Dual-color assay for Smad1–Smad5 and Smad2–Smad3 interactions using different fragments of luciferases.

Figure 4

(A) Ligand-induced Smad1–Smad5 and Smad2–Smad3 interactions. COS-7 cells were cotransfected with CBRN-Smad1, Smad5-McLuc1, ELucN-Smad2 and Smad3-McLuc1 and their bioluminescence intensities were obtained with a luminometer. (*P<0.05) (B) Analysis of bioluminescence with different combination of Smads. COS-7 cells were cotransfected with either CBRN-Smad1 and Smad3-McLuc1 or ELucN-Smad2 and Smad5-McLuc1. Representative data were shown.