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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Inorg Biochem. 2009 Apr 17;103(6):912–922. doi: 10.1016/j.jinorgbio.2009.04.002

Figure 5.

Figure 5

Tyrosyl radical EPR spectra during reaction of the meclofenamate complex of hPGHS-1 with EtOOH. hPGHS-1 (29 μM heme) pre-treated with 2 equivalents of meclofenamate in 100 mM KPi, pH 7.2, 0.1% Tween-20, was reacted on ice with 5 equiv of EtOOH for ~10 s, frozen, and the first EPR spectrum was recorded. For recording subsequent spectra, the sample was thawed and allowed to react at 0 °C for ~10 s before being re-frozen. The arrow indicates the direction of amplitude changes.