Table 2.
Relative Expression of Various mRNAs within FACS-Separated hrGFP-Positive versus hrGFP-Negative Gastric Mucosal Cells of the hrGFP10 Line#
| hrGFP-enriched pools | hrGFP-negative pools | P value | |
|---|---|---|---|
| ghrelin | 5420.67±1097.30 | 1.36±0.24 | 0.0159* |
| chromogranin A | 76.49±16.54 | 1.86±0.73 | 0.0190* |
| prohormone convertase 1/3 | 3.84±0.99 | 0.07±0.04 | 0.0319* |
| prohormone convertase 2 | 4.48±1.21 | 0.10±0.04 | 0.0338* |
| amylase 2 | 1.64±1.26 | 0.13±0.06 | 0.2977 |
| H+/K+ ATPase β-subunit | 2.06±0.78 | 0.88±0.12 | 0.1948 |
| gastric intrinsic factor | 1.04±0.24 | 1.62±0.22 | 0.1965 |
| pepsinogen F | 0.39±0.12 | 0.52±0.21 | 0.6626 |
| calpain 8 | 0.10±0.02 | 0.07±0.02 | 0.2872 |
| histidine decarboxylase | 4.62±2.89 | 0.22±0.14 | 0.2085 |
| somatostatin | 25.70±20.07 | 5.26±2.83 | 0.3216 |
| tryptophan hydroxylase 1 | 0.12±0.05 | 0.16±0.10 | 0.7053 |
| gastrin | 14.15±3.71 | 26.97±8.34 | 0.2208 |
#
all values are normalized to the levels of the housekeeping gene cyclophilin
*
P<0.05 was considered statistically significant