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. Author manuscript; available in PMC: 2010 Jun 12.
Published in final edited form as: Mol Cell. 2009 Jun 12;34(5):545–555. doi: 10.1016/j.molcel.2009.04.018

Figure 2. Transcriptional interference between PR and PRE.

Figure 2

(A) Activity of PRE.(PR−).lacZ and PRE.(PR+).lacZ (n=14), demonstrating a 5.5-fold repression of PRE due to TI from PR. Lines are Hill functions of best fit; the fitted EC50s are 78μM IPTG for PRE.(PR−) and 76μM IPTG for PRE.(PR+).

(B) Activity of PR (short).lacZ (n=6), PR.(PRE+).lacZ (n=15), and pBla.lacZ (n=10), in response to IPTG induction of CII. Only PR.(PRE+).lacZ contains a CII binding site or CII-activated promoter in between the promoter and lacZ.